This paper examines two questions related to autopoiesis as a theory for minimal life: (i) the relation between autopoiesis and cognition; and (ii) the question as to whether autopoiesis is the necessary and sufficient condition for life. First, we consider the concept of cognition in the spirit of Maturana and Varela: in contradistinction to the representationalistic point of view, cognition is construed as interaction between and mutual definition of a living unit and its environment. The most direct form of cognition for a cell is thus metabolism itself, which necessarily implies exchange with the environment and therefore a simultaneous coming to being for the organism and for the environment. A second level of cognition is recognized in the adaptation of the living unit to new foreign molecules, by way of a change in its metabolic pattern. We draw here an analogy with the ideas developed by Piaget, who recognizes in cognition the two distinct steps of assimilation and accommodation. While assimilation is the equivalent of uptake and exchange of usual metabolites, accommodation corresponds to biological adaptation, which in turn is the basis for evolution. By comparing a micro-organism with a vesicle that uptakes a precursor for its own self-reproduction, we arrive at the conclusion that (a) the very lowest level of cognition is the condition for life, and (b) the lowest level of cognition does not reduce to the lowest level of autopoiesis. As a consequence, autopoiesis alone is only a necessary, but not sufficient, condition for life. The broader consequences of this analysis of cognition for minimal living systems are considered.

In this paper we develop the autopoietic approach to the definition of the living developed by Maturana and Varela in the Seventies. Starting from very simple observations concerning the phenomenology of life, we propose a reformulation of the autopoietic original definition of life which integrates some of the contemporary criticism to it. Our definitional proposal, aiming to stimulate the further development of the autopoietic approach, expresses what remains implicit in the definition of the living originally given by Maturana and Varela: life, as self-production, is a process of cognitive coupling with the environment.

Key words: autonomy, autopoiesis, cognition, life, structural coupling

Abstract: The notion of autopoiesis, as originally formulated in the seventies by Maturana and Varela, is first reviewed and it is pointed out how this view offers a definition of minimal life which differs in some respect from the more conventional view, which is based on the template recognition mechanism of nucleic acids. The main criterium for autopoiesis is self-maintenance of the autopoietic unity from within its own. Here it is shown, however, that self-reproduction, when it is originated from reactions taking place within the boundary of the autopoietic unity, can also be seen as a criterium for autopoiesis. Examples of self-reproducing micelles are then reviewed and it is discussed to what extent they can be considered as autopoietic unities. One experiment is discussed in some more detail, as it bears a relation with the origin of life: this is the case of caprylate micelles in water which are spontaneously build from the hydrolysis of water-insoluble ethylcaprylate (EC) – once the first micelles are formed, there is a fast autocatalytic hydrolysis of further EC which produces more micelles in a superexponentially accelerated process. The plan for future experiments in the area, e.g. core-and-shell-replication, is there also outlined. It is finally argued that the progress of this work on autopoietic structures, more than on micelles, must be based on vesicles and liposomes, and preliminary work on this field is discussed.

The concept of autopoiesis was proposed 40 years ago as a definition of a living being, with the aim of providing a unifying concept for biology. The concept has also been extended to the theory of knowledge and to different areas of the social and behavioral sciences. Given some ambiguities of the original definitions of autopoiesis, the concept has been criticized and has been interpreted in diverse and even contradictory ways, which has prevented its integration into the biological sciences where it originated. Here I present a critical review and conceptual analysis of the definition of autopoiesis, and propose a new definition that is more precise, clear, and concise than the original ones. I argue that the difficulty in understanding the term lies in its refined conceptual subtlety and not, as has been claimed by some authors, because it is a vacuous, trivial or very complex concept. I also relate the concept of autopoiesis to the concepts of closed systems, boundaries, homeostasis, self-reproduction, causal circularity, organization and multicellularity. I show that under my proposed definition the concept of a molecular autopoietic system is a good demarcation criterion of a living being, allowing its general integration into the biological sciences and enhancing its interdisciplinary use.

Key words: Autopoiesis, Definition of life, Individual identity, Physical proximity, Self-reproduction, Circular causality

I review here my personal and scientific interactions with Francisco Varela, starting from our meeting in 1983 in Alpbach, Austria, a momentous meeting, which was also the place where the Mind and Life Institute and independently the Cortona week were conceived. Later on, the scientific cooperation focussed on autopoiesis and permitted to arrive at the experimental autopoiesis on the basis of the self-reproduction of micelles and vesicles. I then briefly describe how Francisco, based on the complementary notion of cognition, was able to draw the bridge between biology and cognitive sciences. The main keywords here are enaction and embodied mind. From here, and towards the end of his life, Francisco focussed mostly on neurobiology, where he introduced the notion of neurophenomenology centred on first-person reports. However, his seminal work on autopoiesis was instrumental to conceive the new field of research on the minimal cells, which is briefly described. I conclude with an overview of the meaning of the work of Francisco for life sciences at large.

Key words: Mind and Life Institute, autopoiesis, enaction, embodied mind, neurophenomenology, life sciences

Reverse micelles hosting the internal production of the surfactant are proposed as experimentally feasible models of simple (or ‘minimal’) autopoietic systems. We describe the conditions under which these may be formed and their possible biological implications. The micellar systems considered here turn out also to exhibit a capacity for self-reproduction through fragmentation under plausible conditions, thus constituting also a minimal experimental model for prebiotic self-reproduction.

Spherical bounded structures such as those formed by surfactant aggregates (mostly micelles and vesicles), with an inside that is chemically and physically different from the outside medium, can be seen as primitive cell models. As such, they are fundamental structures for the theory of autopoiesis as originally formulated by Varela and Maturana. In particular, since self-reproduction is a very important feature of minimal cellular life, the study of self-reproduction of micelles and vesicles represents a quite challenging bio-mimetic approach. Our laboratory has put much effort in recent years into implementing self-reproduction of vesicles as models for self-reproduction of cellular bounded structures, and this article is a further contribution in this direction. In particular, we deal with the so-called matrix effect of vesicles, related to the fact that when fresh surfactant is added to an aqueous solution containing preformed vesicles of a very narrow size distribution, the newly formed vesicles (instead of being polydisperse, as is usually the case) have dimensions very close to those of the preformed ones. In practice, this corresponds to a mechanism of reproduction of vesicles of the same size. In this article, the matrix effect is re-elaborated in the perspective of the origin of life, and in particular in terms of the prebiotic mechanisms that might permit the growth and reproduction of vesicles. The data are analyzed by dynamic light scattering with a new program that permits the calculation of the number-weighted size distribution. It is shown that, on adding a stoichiometric amount of oleate micelles to preformed oleate vesicles extruded at 50 and 100 nm, the final distribution contains about twice the initial number of particles, centered around 50 and 100 nm. The same holds when oleate is added to preformed phospholipid liposomes. By contrast, when the same amount of oleate is added to an aqueous solution (as a control experiment), a very broad distribution ranging between 20 and 1000 nm is obtained. The data can then be seen as a kind of reproduction of the same size vesicles, and the argument is advanced that this may correspond to a simple prebiotic mechanism of vesicle multiplication in prebiotic times, when only physical forces might be responsible for the basic mechanisms of early protocell growth and division. Preliminary data also show that repeated addition of oleate maintains the same basic initial features, and that surfactants other than oleate also respect the reproductive mode of the matrix effect.

Context: At the conference “The Ethical Meaning of Francisco Varela’s Thought,” which took place on 28 May 2011 in Sassari, Italy, Humberto Maturana, Michel Bitbol, and Pier Luigi Luisi participated in two discussions. Purpose: In this edited transcription of the discussions, the participants talk about several aspects of autopoiesis, the observer, ontology, making distinctions and distinguishing different domains, perception and illusion, and transcendence. Results: The discussions shed light on how constructivist concepts are perceived by individual authors. Concepts such as “transcendence” and “objectivity” are understood in different ways. Constructivist content: The concepts discussed are highly relevant for constructivist approaches.

Key words: Autopoiesis, observer, ontology, distinctions, objectivity, transcendence